Pichia Pastoris: a Platform Organism to Produce Proteins
نویسنده
چکیده
Contents 1. Introduction 2. The P. pastoris expression system 2.1. The AOX1 promoter and alternative promoters 2.1.1 AOX1 promoter. 2.1.2. Strongly expressed alternative promoters. 2.1.3. Moderately expressed alternative promoters. 2.2. Host strains 2.2.1. Methanol utilization phenotype 2.2.2. Protease-deficient host strains 2.3. Expression vectors 3. Bioreactor process techniques for production of recombinant proteins with P. pastoris 3.1 Bioreactor fed-batch technique 3.2 Standard P. pastoris bioreactor fermentation technique 3.2.
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